Long-chainn-3 polyunsaturated fatty acid from fish oil modulates aortic nitric oxide and tocopherol status in the rat

Resumen

In spite of their high oxidisability, long-chainn-3 PUFA protect against CVD. Dietary fatty acids modulate the fatty acid composition of lipoproteins involved in atherosclerosis. We thought that if long-chainn-3 PUFA were able to increase NO production by the aorta, then by its antioxidant activity the NO will prevent lipid peroxidation. However, the beneficial effect of NOin vivoon VLDL+LDL oxidation would only be possible if NO could diffuse to their lipidic core. Rats were fed maize oil- or fish oil as menhaden oil- (MO) rich diets for 8 weeks, to study the effects of MO on aortic NO production, NO diffusion into VLDL+LDL, the extent of oxidation in native VLDL+LDL and their oxidisabilityex vivo. Aortic NO production and its α-tocopherol content were increased andn-3 PUFA were incorporated into the VLDL+LDL. In spite of the higher peroxidisability and the low α-tocopherol in native VLDL+LDL from rats fed MO, native VLDL+LDL from the two groups shared similar electrophoretic patterns, conjugated dienes, thiobarbituric acid-reactive substances, total antioxidant capacity, and NO diffusibility on VLDL+LDL, indicative of anin vivoprotection against oxidation. However, these results do not correlate with theex vivooxidisability of VLDL+LDL, as NO is lacking. Thus, thein vivobeneficial effects can be explained by increased α-tocopherol in aorta and by a compensatory effect of NO on VLDL+LDL against the low α-tocopherol levels, which may contribute to the anti-atherogenic properties of fish oil.